施马伦贝格病毒病焦磷酸测序检测方法的建立

doi:10.11843/j.issn.0366-6964.2015.01.022

Abstract

Abstract:

The aim of the present study was to establish a pyrosequencing method targeting S gene for detection and identification of schmallenberg virus (SBV).In alignment with published more than 10 viruses S gene sequences which belong to Bunyaviridae and are highly homogenous with SBV.Adaptive sequence for pyrosequencing was synthesized and in vitro transcripted to cRNA as the RT-PCR templates.After RT-PCR reaction，the products were detected with forward and reverse pyrosequencing and identified by genomic alignment.Results showed that the RT-PCR products were 166 bp.The sensitivity of the method that the minimum copies could be detected was 104 copies.The specificity that sequencing length by pyrosequencing was around 100 bp which could make a definite diagnosis for SBV.The results presented here demonstrate that the detection and identification method for SBV by pyrosequencing was established.The method can be used in fast diagnosis for SBV.

CLC Number:

S852.659.5

ZHANG Yong-qiang，WU Xiao-dong，ZHAO Yong-gang，WANG Zhi-liang. Establishment of Detection and Identification Method for Schmallenberg Virus by Pyrosequencing[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(1): 174-178.

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Establishment of Detection and Identification Method for Schmallenberg Virus by Pyrosequencing

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